with flowcytometry, cytogenetic and molecular biology findings
Novartis IBA FM MU

B-lymphoblastic leukaemia/lymphoma with t(9;22)(q34.1;q11.2); BCR-ABL1

G-banded male karyotype with t(9;22)(q34;q11.2)

G-banded karyotype 46,XY,t(9;22)(q34;q11.2) of a bone marrow metaphase cell from an ALL patient. The reciprocal translocation t(9;22)(q34;q11.2) results into derivative chromosomes der(22) (Philadelphia chromosome) [red arrow] and der(9) [yellow arrow].

G-banded partial karyotype of translocation t(9;22)(q34;q11.2)

G-banded partial karyotype demonstrating the translocation t(9;22)(q34;q11.2). Red arrows indicate derivative chromosomes 9 and 22. Breakpoint sites are indicated by black arrows on normal chromosome homologues.

Fluorescence in situ hybridisation (FISH) of BCR-ABL1 fusion

Fluorescence in situ hybridisation (FISH) of the BCR-ABL1 fusion performed on (A) metaphase chromosomes and (B) an interphase cell [a dual-colour, dual-fusion FISH probe was used]. Red arrows indicate the chromosomal region 9q34, where the ABL1 gene is located, whereas green arrows indicate the chromosomal region 22q11.2, where the BCR gene is located. Yellow arrows indicate fusion signals (red-green) of genes BCR-ABL1 on the Philadelphia chromosome and on the derivative chromosome 9 with the ABL1-BCR fusion (A) and on an interphase cell (B).

Atlas of Haematological Cytology [online]. 2016 [cit. 2024-3-28]. Available from WWW: http://www.leukemia-cell.org/atlas.

2024 CELL - Atlas of Haematological Cytology | site map